PeproGrow™ EPC 內皮祖細胞培養(yǎng)基

英文名：PeproGrow™ EPC (Endothelial Progenitor Cell Media)

中文名：PeproGrow 內皮祖細胞培養(yǎng)基

PeproGrow EPC 適用培養(yǎng)內皮祖細胞。能良好維持內皮祖細胞的形態(tài)和功能，并提高內皮一氧化氮合酶活性。

產品訂購信息

產品描述

PeproTech提供三種內皮細胞培養(yǎng)基，分別適用于體外培養(yǎng)：骨髓或外周血來源的內皮祖細胞（PeproGrow™ EPC ），大血管內皮細胞（PeproGrow™ MacroV）和微血管內皮細胞（PeproGrow™ MicroV）。這些培養(yǎng)基可以維持良好的內皮細胞形態(tài)和功能，并能提高內皮細胞關鍵的特異性標志物——內皮一氧化氮合酶（eNOS）的活性。由此，它們可為大血管、微血管內皮細胞及EPCs提供優(yōu)良的細胞培養(yǎng)環(huán)境，且細胞生長率高于目前市售的其他培養(yǎng)基。

Product Details

Catalog Number:700-EPC

Description:

PeproTech offers three separate endothelial cell culture media formulations developed for the in vitro c*tion of: endothelial progenitor cells (EPC; PeproGrow EPC) derived from bone marrow or peripheral blood; endothelial cells from large vessels (PeproGrow MacroV); and endothelial cells from small vessels (PeproGrow MicroV). These media formulations maintain outstanding endothelial cell morphology and function, and increase the activity of endothelial nitric oxide synthase (eNOS), which account for a specific, crucial marker for endothelial cells. By doing this, the media provide an optimal cell culture environment for macrovascular and microvascular endothelial cells, as well as for EPC; growing cells at rates that exceed commercially available media. These media formulations are optimized for primary/progenitor human cells, but can also be used for bovine, porcine, rabbit, rat, and mouse EPC, or mature endothelial cells. 

PeproGrow EPC is supplied as a 500mL bottle of basal medium and a separate, frozen growth factor component.

PeproGrow EPC適用

PeproGrow EPC is recommended for Endothelial Progenitor Cells:

PeproGrow EPC推薦內皮祖細胞培養(yǎng)：
Human Endothelial Progenitor Cells (hEPC) 人內皮祖細胞
Mouse Endothelial Progenitor Cells (mEPC)  小鼠內皮祖細胞

PeproGrow EPC特征
Figures 1 and 2: EPC Proliferation. EPC were seeded onto fibronectin-coated plates, and incubated for 7 days in PeproGrow EPC basal medium + Growth Supplement-EPC (PeproTech) and a competitor’s medium. The proliferation ability of EPC was assessed 7 days after cell c*tion using the XTT assay according to the manufacturer protocol. The proliferation ability of EPC was expressed as the average of fluorescence intensity (O.D.) calculated using a plate reader for two independent assays run in triplicates. 

圖一 EPC 擴增：左 其他品牌；右 Peprotech

內皮祖細胞擴增。內皮祖細胞接種在人纖連蛋白包被培養(yǎng)板上，分別使用PeproGrow EPC基礎培養(yǎng)基+EPC生長因子（PeproTech）和其他品牌的培養(yǎng)基孵育7天。在細胞培養(yǎng)后7天用XTT分析培養(yǎng)基的內皮祖細胞擴增能力。

儲存：基礎培養(yǎng)基保存在2-8°C。生長因子使用前保存在 -20°C。

內皮一氧化氮合酶介紹

內皮一氧化氮合酶是一種一氧化氮合成酶。其他還有神經一氧化氮合酶和誘生性一氧化氮合酶。內皮一氧化氮合酶主要作用于血管內皮細胞的NO生成。NO對血管張力調節(jié)，細胞擴增，淋巴細胞粘連，血小板凝集都有重要作用。因此內皮一氧化氮合酶功能是健康心血管系統的基礎。（英文：Wiki）

其他內皮細胞培養(yǎng)基

ScienCell 1001 內皮細胞培養(yǎng)基 現貨供應（超鏈接）

PeproTech 內皮細胞培養(yǎng)基系列

PeproTech提供三種內皮細胞培養(yǎng)基試劑盒，分別適用于體外培養(yǎng)：骨髓或外周血來源的內皮祖細胞（PeproGrow™ EPC ），大血管內皮細胞（PeproGrow™ MacroV）和微血管內皮細胞（PeproGrow™ MicroV）。每款試劑盒由基礎培養(yǎng)基和一管單獨的內皮細胞生長添加劑組成。

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